Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2006, Vol. 17 ›› Issue (5): 280 doi: 10.1016/S1001-8042(06)60052-3

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Cytotoxicity of fullerenols on Tetrahymena pyriformis

ZHAO Qun-Fen1,2 ZHU Ying1 RAN Tie-Cheng1 LI Jun-Gang1 LI Qing-Nuan1 LI Wen-Xin1,*   

  1. 1Laboratory of Nano, Biology and Medicine, Shanghai Institute of Applied Physics, the Chinese Academy of Sciences, Shanghai 201800, China;
    2 Faculty of Life Science and Bioengineering, Ningbo University, Ningbo 315211, China
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ZHAO Qun-Fen ZHU Ying RAN Tie-Cheng LI Jun-Gang LI Qing-Nuan LI Wen-Xin. Cytotoxicity of fullerenols on Tetrahymena pyriformis.Nuclear Science and Techniques, 2006, 17(5): 280     doi: 10.1016/S1001-8042(06)60052-3
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Abstract: With the increasing use of fullerenes and their derivatives in a variety of fields, the toxicity and effects of fullerenes on humans and the environment have received considerable attention. In this study, the cytotoxicity of fullerene derivative, C60(OH)x, on Tetrahymena pyriformis was investigated. Cell growth inhibition was evaluated by counting with an optical microscope, and the generation time was calculated. It was indicated that the fullerenols caused a dose-dependent growth inhibition of the cells. The morphologic change in the damaged macronucleus of cells was observed using a fluorescent microscope. Superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and glutathione reductase (GR) levels were also measured for Tetrahymena pyriformis, using conventional methods. The results showed that fullerenols could reduce GSH-PX and GR activities. But no noticeable difference in SOD activity was observed between the treated groups and the control group. This indicated that the antiproliferative effect of fullerenols might be mediated by the reduction in the activities of GSH-PX and GR of cells and the destruction of the macronucleus.

Key words: Fullerenol, Cytotoxity, Tetrahymena pyriformis