Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2019, Vol. 30 ›› Issue (2): 25 doi: 10.1007/s41365-019-0554-x


Synchrotron infrared spectral regions as signatures for foodborne bacterial typing

Ya-Di Wang1,2 • Xue-Ling Li3 • Jun Hu1 • Jun-Hong Lü1   

  1. 1 Division of Physical Biology and CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences (CAS), Shanghai 201800, China
    2 University of Chinese Academy of Sciences, Beijing 100049, China
    3 Shanghai Center for Bioinformation Technology, Shanghai Industrial Technology Institute, Shanghai 201203, China
  • Received:2018-07-15 Revised:2018-08-25 Accepted:2018-09-01
  • Contact: Xue-Ling Li; Jun-Hong Lü;
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (Nos. U1732130 and 11474298) and Key Research Program of Frontier Sciences of the Chinese Academy Sciences (No. QYZDJSSW- SLH019).
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Ya-Di Wang, Xue-Ling Li, Jun Hu, Jun-Hong Lü. Synchrotron infrared spectral regions as signatures for foodborne bacterial typing.Nuclear Science and Techniques, 2019, 30(2): 25     doi: 10.1007/s41365-019-0554-x

Abstract: Fourier-transform infrared (FTIR) spectroscopy has emerged as a viable alternative to biochemical and molecular biology techniques for bacterial typing with advantages such as short analysis time, low cost and laboratorial simplicity. In this study, synchrotron radiationbased FTIR (SR-FTIR) spectroscopy with higher spectral quality was successfully applied to type 16 foodborne pathogenic bacterial strains. Combined with principal component analysis (PCA) and hierarchical cluster analysis (HCA), we found that the specific spectral region 1300–1000 cm-1, which reflects the information of phosphate compounds and polysaccharides, can be used as the signature region to cluster the strains into groups similar with genetic taxonomic method. These findings demonstrated that FTIR spectra combined with HCA have a great potential in quickly typing bacteria depending on their biochemical signatures.

Key words: Synchrotron radiation, FTIR, Spectral signature, Bacterial typing, PCA, HCA