Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2014, Vol. 25 ›› Issue (3): 030502 doi: 10.13538/j.1001-8042/nst.25.030502

• NUCLEAR PHYSICS AND INTERDISCIPLINARY RESEARCH • Previous Articles     Next Articles

Using Xe as a heavy atom for phase determination of protein trichosanthin structure

CHEN Ya-Xing, LI Min-Jun, YU Feng, LIU Ke, XU Chun-Yan, SUN Bo, ZHOU Huan, XU Qin, HE Jian-Hua   

  1. 1Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 239 Zhangheng Road, Shanghai 201204, China
  • Contact: HE Jian-Hua E-mail:liminjun@sinap.ac.cn; hejianhua@sinap.ac.cn
  • Supported by:

    Supported by the Major State Basic Research Development Project in
    China (No. 2011CB911102) and National Natural Science Foundation of China (Nos. 31000389, 31100528 and 31100529)

CHEN Ya-Xing, LI Min-Jun, YU Feng, LIU Ke, XU Chun-Yan, SUN Bo, ZHOU Huan, XU Qin, HE Jian-Hua. Using Xe as a heavy atom for phase determination of protein trichosanthin structure.Nuclear Science and Techniques, 2014, 25(3): 030502     doi: 10.13538/j.1001-8042/nst.25.030502

Abstract:

Under gas pressures of 1–3MPa, xenon can be bound to discrete sites in hydrophobic cavities of protein, so as to use Xe as a heavy atom for determining phases in protein crystallography. Using single anomalous scattering diffraction method, we demonstrate that an interpretable electron density map can be obtained for protein trichosanthin from a single xenon derivative. For the first time, we found a pre-existing hydrophobic cavity just under the protein surface of trichosanthin.

Key words: Xenon, Phasing, SAD, Hydrophobic cavity, Trichosanthin