Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2013, Vol. 24 ›› Issue (6): 060304 doi: 10.13538/j.1001-8042/nst.2013.06.015

• NUCLEAR CHEMISTRY,RADIOCHEMISTRY,RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE • Previous Articles     Next Articles

Alterations of mtDNA copy number and 4977 bp deletion induced by ionizing radiation in human peripheral blood

LIU Yulong 2,** WANG Ping 1, ** HAN Lin 1 ZHAO Fengling 1 GUO Fei 3#br# WANG Xiai 1 LV Yumin 1,3,*   

  1. 1Henan Institute of Occupational Medicine, Zhengzhou 450052, China
    2The Second Affiliated Hospital of Soochow University, Soochow 215004, China
    3College of Public Health, Zhengzhou University, Zhengzhou 450001, China
  • Contact: LIU Yulong E-mail:lym636160@sina.com
  • Supported by:

    Supported by grants from the building project for the National Key Clinical Special Department of China (No.2011-17), the Medical Science and Technology Foundation of Henan Province (No.201204123), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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LIU Yulong, WANG Ping, HAN Lin, ZHAO Fengling, GUO Fei. Alterations of mtDNA copy number and 4977 bp deletion induced by ionizing radiation in human peripheral blood.Nuclear Science and Techniques, 2013, 24(6): 060304     doi: 10.13538/j.1001-8042/nst.2013.06.015

Abstract:

Alterations of mitochondria DNA (mtDNA) 4977 bp common deletion (CD) and mtDNA copy number induced by ionizing radiation were observed in human different cell lines and total body irradiation patients. However, only few experiments have evaluated the levels of the CD and mtDNA copy number in human peripheral blood exposed to ionizing radiation till now. The aim of this study is to analyze the mtDNA alterations in irradiated human peripheral blood from healthy donors as well as to explore their feasibility as biomarkers for constructing new biodosimeter. Peripheral blood samples were collected from six healthy donors, and exposed to 60Co gamma ray with the doses of 0 Gy, 1 Gy, 2 Gy, 3 Gy, 4 Gy and 5 Gy. Levels of the CD and mtDNA copy number in irradiated samples after 2h or 24h incubation were detected using TaqMan real-time PCR, and the CD ratio was calculated. The results showed that the mean of the CD ratio and the CD copy number exhibited a dose-dependent increase 2 h in the dose range from 0–5 Gy, and of the mtDNA copy number significantly increased 24 h in irradiated groups compared with 0 Gy group after irradiation. It indicates that the parameters in human peripheral blood may be considered as molecular biomarkers to applying construction of new biodosimeter.

Key words: Ionizing radiation, Human peripheral blood, Mitochondria DNA, 4977 bp common deletion, TaqMan real-time PCR