Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2015, Vol. 26 ›› Issue (5): 050504 doi: 10.13538/j.1001-8042/nst.26.050504


Purification of rectangle DNA origami by rate-zonal centrifugation

CHAO Jie,1, 2 XING Shu, 2 FENG Bian-Ying, 2 WANG Jian-Bang, 2 DAI Jiang-Bing, 2 WANG Li-Hua, 2 LI Qian 2   

  1. 1Key Laboratory for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), and School of Materials Science and Engineering, Nanjing University of Posts & Telecommunications, Nanjing 210046, China
    2Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
  • Contact: LI Qian
  • Supported by:

    Supported by Shanghai Natural Science Foundation (Nos. 15ZR1448400
    and 15ZR1448700) and National Natural Science Foundation of China
    (No. 31300825)

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CHAO Jie, XING Shu, FENG Bian-Ying, WANG Jian-Bang, DAI Jiang-Bing, WANG Li-Hua, LI Qian . Purification of rectangle DNA origami by rate-zonal centrifugation.Nuclear Science and Techniques, 2015, 26(5): 050504     doi: 10.13538/j.1001-8042/nst.26.050504


DNA origami technique, a breakthrough in DNA nanotechnology, has been widely used to prepare complex DNA nanostructures with nanoscale addressability. However, the purity and yield are generally the bottleneck to application of DNA nanostructures, and current methods for purifying DNA origami nanostructures in large quantities are time-consuming and laborious. This study aims to develop a scalable, cost-effective and contamination-free method of purifying DNA origami nanostructures. We employ an effective and convenient purification approach to purify planar rectangle DNA origami structures through rate-zonal centrifugation. By subjecting DNA origami samples to high centrifugal force in a density gradient media of glycerol, well-folded nanostructures and by-products are separated successfully, which are confirmed by agarose gel electrophoresis and atomic force microscopy (AFM). This method will aid the production of pure rectangle DNA origami nanostructures in large quantity.

Key words: DNA origami, Purification, Rate-zonal centrifugation, Glycerol gradient