Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2016, Vol. 27 ›› Issue (5): 112 doi: 10.1007/s41365-016-0117-3


Flower color mutants induced by carbon ion beam irradiation of geranium (Pelargonium 3 hortorum, Bailey)

Li-Xia Yu 1,2 , Wen-Jian Li 1 , Yan Du , Gang Chen 3 , Shan-Wei Luo 2 , Rui-Yuan Liu 1,4 , Hui Feng 2 , Li-Bin Zhou 1   

  1. 1 Biophysics Group, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000, China
    2 University of Chinese Academy of Sciences, Beijing 100049, China
    3 Ningxia Academy of Agriculture and Forestry Sciences, 590 West Huanghe, Yinchuan 750002, China
    4 Gansu Agricultural University, No. 1 Yingmen Village, Anning District, Lanzhou 730070, China
  • Contact: Li-Bin Zhou
  • Supported by:

    This work is supported by the National Natural Science Foundation of China (Grant Nos. 11205218, 11275171, and 11405234), the Knowledge Innovation Project of the Chinese Academy of Sciences (CAS) (No. KJCX2-EW-N05), CAS ‘‘Light of West China’’ Program (No. 29Y506020), and the Youth Innovation Promotion Association of CAS (No. 29Y506030) supported this study.

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Li-Xia Yu, Wen-Jian Li, Yan Du, Gang Chen, Shan-Wei Luo, Rui-Yuan Liu, Hui Feng, Li-Bin Zhou. Flower color mutants induced by carbon ion beam irradiation of geranium (Pelargonium 3 hortorum, Bailey).Nuclear Science and Techniques, 2016, 27(5): 112     doi: 10.1007/s41365-016-0117-3


In an attempt to elucidate the biological effects and underlying mutations involving flower color in ornamental plants following carbon ion beam radiation, shoots of geranium were exposed at dosages of 0, 10, 15, 30, and 40 Gy, and one flower color mutant was obtained. The morphological characteristics, physiological aspects, and DNA polymorphisms between wild-type and flower color mutants were analyzed. The colors of petal, peduncle, pistil, and stamen of the mutant displayed significant differences compared to those of the wild-type. Compared to the original plants, the total anthocyanin content in the petals of the mutant significantly decreased, resulting in a light pink petal phenotype. DNA polymorphisms detected by random amplified polymorphic DNA analysis showed that the ratio of different bands between the wild-type and mutant reached up to 13.2 %. The present study demonstrates that carbon ion beam irradiation is effective in inducing genomic variations, resulting in flower color geranium mutants within a relatively short period of time. Meanwhile, the developed flower-color mutants may be potentially used in future mutational research studies involving ornamental plants.

Key words: Carbon ion beams, Geranium, Flower color mutant, Mutation breeding