Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2017, Vol. 28 ›› Issue (1): 1 doi: 10.1007/s41365-016-0157-8

• NUCLEAR CHEMISTRY,RADIOCHEMISTRY,RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE •     Next Articles

A high-throughput screening method for breeding Aspergillus niger with 12C6+ ion beam-improved cellulase

Bo-Ling Jiang 1,2, Shu-Yang Wang 1,3, Yu-Chen Wang 1,4, Ji-Hong Chen 1, Wen-Jian Li 1, Jing Liu1, Wei Hu1, Guo-Qing Xiao1, Miao-Ying Dong1, Fu-Qiang Xu1   

  1. 1 Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd, Lanzhou 730000, China
    2 University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China
    3 Lanzhou University, 222 South Tianshui Road, Lanzhou 730000, Gansu Province, China
    4 Gansu Agricultural University, Lanzhou 730000, Gansu Province, China
  • Contact: Shu-Yang Wang E-mail:wangsy@impcas.ac.cn
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (No. 11305225), the Science and Technology Service Network Initiative (KFJ-EW-STS-086).

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Bo-Ling Jiang, Shu-Yang Wang, Yu-Chen Wang, Ji-Hong Chen, Wen-Jian Li, Jing Liu, Wei Hu, Guo-Qing Xiao, Miao-Ying Dong, Fu-Qiang Xu. A high-throughput screening method for breeding Aspergillus niger with 12C6+ ion beam-improved cellulase.Nuclear Science and Techniques, 2017, 28(1): 1     doi: 10.1007/s41365-016-0157-8
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Abstract:

In this study, a high-throughput screening method was established through the 24-square deep-well microliter plate (MTP) fermentation and micro-plate detection for large-scale screening of the mutants. It was suitable for screening a large number of mutants and improving the breeding efficiency after heavy-ion beam irradiation. Seventeen strains showed higher cellulase activity compared with the initial strain after the screening of plate and MTP fermentation. The filter paper activity and β-glucosidase activity of Aspergillus niger H11201 had increased 38.74 and 63.23 % separately compared with A. niger H11 by shaking flask fermentation, and it was genetically stable after being passaged to nine generations. The results indicate that the high-throughput screening method can be used for the quick breeding of A. niger with high cellulase activity.

Key words: High-throughput screening method, Aspergillus niger H11, Cellulase, 12C6+ ion beam