Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2018 Impact factor 0.961

Nuclear Science and Techniques ›› 2008, Vol. 19 ›› Issue (6): 347-353

• RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICIN • Previous Articles     Next Articles

Preparation and preliminary evaluation of [55Co](II)vancomycin

Amir Reza JALILIAN * Yousef YARI-KAMRANI Pejman ROWSHANFARZAD Mahseed SABET Mohsen KAMALI-DEHGHAN Abbas MAJDABADI   

  1. Nuclear Medicine Research Group, Agriculture, Medicine and Industrial Research School (AMIRS), Karaj, Iran
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Amir Reza JALILIAN, Yousef YARI-KAMRANI, Pejman ROWSHANFARZAD. Preparation and preliminary evaluation of [55Co](II)vancomycin.Nuclear Science and Techniques, 2008, 19(6): 347-353

Abstract: Co-55 (t1/2=17.53 h) was produced by 150 μA irradiation of a natural nickel target using 15 MeV protons. It was separated from the irradiated target material by two ion exchange chromatography steps with a radiochemical yield of >95% and was used for the preparation of [55Co]vancomycin ([55Co]VAN). Optimization studies were performed using Co-57 due to its longer half-life. Cobalt-57 (t1/2=271.79 d) was produced by irradiation of a natural nickel target with 150 μA current of 22 MeV protons. The 57Co was separated from the irradiated target material using a no-carrier-added method with a radiochemical yield of >97%. Both products were controlled for radionuclide and chemical purity. The solutions of [55Co]VAN were prepared (radiochemical yield>80%) starting with 55Co acetate and vancomycin at room temperature after 30 min. A precise solid phrase extraction (SPE) method was developed using Si Sep-Pak in order to purify/reconstitute the final formulation for animal studies. [55Co]VAN showed a radiochemical purity of more than 99%. The resultant specific activity was about 1.15 TBq/mmol. It is proved that the tracer is stable in the final product and in presence of human serum at 37°C up to 24 h. Biodistribution study of [55Co]VAN in normal rats was undertaken for up to 72 h.

Key words: Cobalt-55, Vancomycin, Medical cyclotron, Radiolabeling, Biodistribution