Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2018 Impact factor 0.961

Nuclear Science and Techniques ›› 2004, Vol. 15 ›› Issue (2): 106

• RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICIN • Previous Articles     Next Articles

Studies on apoptosis in bone tumor cells induced by 153Sm

ZHU Shou-Peng, XIAO Dong, HAN Xiao-Feng   

  1. Institute of Radiological Medicine, Soochow University, Suzhou 215007
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ZHU Shou-Peng, XIAO Dong, HAN Xiao-Feng. Studies on apoptosis in bone tumor cells induced by 153Sm.Nuclear Science and Techniques, 2004, 15(2): 106

Abstract: The apoptosis in human bone tumor cells induced by internal irradiation with 153Sm was studied. The morphological changes in bone tumor cells were observed by electronic and fluorescent microscopy, as well as DNA agarose gel eletrophoresis. DNA chain fragmentation, microautoradiographic tracing and the inhibition rate of proliferation in bone tumor cells exposed to 153Sm with different duration time were examined. It was demonstrated that the bone tumor cells exposed to 153Sm displayed nuclear fragmentation, pyknosis, margination of condensed chromatin, and formation of membrane bounded apoptotic bodies, whereas the percentage of DNA chain fragmentation of bone tumor cells increases in direct proportion to the duration of irradiation with 153Sm, as well as DNA ladder formation in apoptotic cells. Also a marked inhibition effect of proliferation in bone tumor cells after exposure with 153Sm was observed

Key words: Apoptosis, Bone tumer cells, 153Sm, Nuclear fragmentation, DNA ladder formation