Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2003, Vol. 14 ›› Issue (1): 69

• RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICIN • Previous Articles     Next Articles

Application of N-succinimidyl 4-[18F](fluoromethyl) benzoate to protein labeling

LI Jun-Ling, WANG Yong-Xian, ZHANG Xiu-Li, ZHOU Wei , TIAN Hai-Bin, YIN Duan-Zhi   

  1. Shanghai Institue of Nuclear Research, the Chinese Academy of Sciences, Shanghai 201800
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LI Jun-Ling, WANG Yong-Xian, ZHANG Xiu-Li, ZHOU Wei , TIAN Hai-Bin, YIN Duan-Zhi. Application of N-succinimidyl 4-[18F](fluoromethyl) benzoate to protein labeling.Nuclear Science and Techniques, 2003, 14(1): 69

Abstract: N-succinimidyl 4-[18F](fluoromethyl) benzoate for protein labeling was prepared (57%, EOB) in about 30min. Reaction conditions of S18FMB with IgG including pH of solutions, protein concentration, reaction temperature and time were studied. The optimal labeling conditions were: 0.2mg/mL IgG, pH = 7.8-8.5, 25℃, and reaction time 5 min. Under these conditions the yield was about 80%. The 18F-labeled protein was purified by size exclusion chromatography.

Key words: S18FMB, IgG, Protein labeling, Size exclusion chromatography