Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2001, Vol. 12 ›› Issue (2): 94

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Preparation and 99mTc labeling of hydrazino nicotinamide modified RC-160 conjugate

HU Ji1, SUZUKI Kazuko Horiuchi2, SAJI Hideo2   

  1. 1 Isotope Department, China Institute of Atomic Energy, Beijing 102413;
    2 Kyoto University's Graduate School of Pharmaceutical Sciences Department of Patho-Functional Bioanalysis, Kyoto, Japan
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HU Ji, SUZUKI Kazuko Horiuchi, SAJI Hideo. Preparation and 99mTc labeling of hydrazino nicotinamide modified RC-160 conjugate.Nuclear Science and Techniques, 2001, 12(2): 94

Abstract: Conjugations of 6-Boc-hydrazinopyridine-3-carboxylie acid (Boc-HYNIC) with RC-160 [Lys5, Boc] using different coupling methods were studied. After deprotection, the resulted peptide conjugates were purified by RP-HPLC and characterized by Fab-MS. Combination with different coligands, 99mTc labeling of HYNIC modified RC-160 conjugate was carried out with high radiolabeling yield (>95%). In vitro stability studies indicated that the final radjo-compound remained stable at least up to 20h when nicotinic acid (NA) used as second coligand (tricine/NA).

Key words: Conjugation, 99mTc labeling, Somatostatin analogue