Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 1999, Vol. 10 ›› Issue (4): 93

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Preparation,characterization and radiolabelling of antigranulocyte monoclonal antibody

GU Zhaoxiang, LIN Xiangtong, XUE Fangping, ZHANG Jinming   

  1. Huashan Hospital, Shanghai Medical University, Shanghai 200040
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GU Zhaoxiang, LIN Xiangtong, XUE Fangping, ZHANG Jinming. Preparation,characterization and radiolabelling of antigranulocyte monoclonal antibody.Nuclear Science and Techniques, 1999, 10(4): 93

Abstract: The human granulocytes were isolated. Using hybridoma techniques, a hybridoma cell line (HSN) producing monoclonal antibody (McAb) against human granulocyte was obtained. The antibody belonged to IgG1 subclass. It was confirmed by immunohistochemical tests that HSN reacted selectively not only with human granulocytes, but also with their bone marrow precursors. Whereas human lymphocytes and red blood cells retained negative in the tests. No cross-reaction was observed with the peripheral blood cells in other animals. Its affinity constant was 5.7×108 L/mol, and the number of epitopes per granulocyte was 4.7×105. Monoclonal antibody displayed no loss of immunoreactivity after labelled with 99mTc.

Key words: Monoclonal antibody, Human granulocytes, Technetium-99m labelling, Hybridoma