Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2018 Impact factor 0.961

Nuclear Science and Techniques ›› 1999, Vol. 10 ›› Issue (1): 44

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A solid phase radioimmunoassay for free triiodothyronine in serum:assay development and validation

LIU Fangyan, ZHOU Meiying, YIN Linxiang, YANG Jianzhong, HUA Chenglin   

  1. Shanghai Institute of Nuclear Research, the Chinese Academy of Sciences, Shanghai 201800
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LIU Fangyan, ZHOU Meiying, YIN Linxiang, YANG Jianzhong, HUA Chenglin. A solid phase radioimmunoassay for free triiodothyronine in serum:assay development and validation.Nuclear Science and Techniques, 1999, 10(1): 44

Abstract: A solid phase radioimmunoassay for free triiodothyronine in serum was developed based on double-antibody coated tubes. The method was turned out to be reliable with good reproducibility, higher sensitivity and easy performance. The measurable range of FT3 in serum was 1.2 to 38 μmol/L. The mean coefficients of variation within and between assays were 1.79%~3.18% and 4.72%~9.31%, respectively. The FT3 concentrations in euthyroid serum as determined by this method were 2.8 to 7.8pmol/L. The FT3 values determined by this new method correlated well with those measured by a commercial radioimmunoassay (r=0.853).

Key words: Free triiodothyronine (FT3), Solid-phase, Radioimmunoassay, T3 analog, Doubleantibody