Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 1997, Vol. 8 ›› Issue (4): 229

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Apoptosis in immune cells induced by fission fragment 147Pm

Zhu Shou-Peng, Zhang Lan-Sheng and Fu Qiang   

  1. Suzhou Medical College, Suzhou 215007
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Zhu Shou-Peng, Zhang Lan-Sheng and Fu Qiang. Apoptosis in immune cells induced by fission fragment 147Pm.Nuclear Science and Techniques, 1997, 8(4): 229

Abstract: Apoptosis in human acute lymphoblastic leukemia cell line Molt-4 cell and macrophage cell line Ana-l cell could be induced by fission fragment 147Pm. The cumulative absorption dose of 147Pm in cultural cells through different periods were estimated. By using fluorescence microscopy and microautoradiographic tracing it can be found that Molt-4 and Ana-l cells internally irradiated by 147Pm, displayed an obvious nuclear fragmentation and a marked pyknosis in immune cell nuclei, as well as DNA chain fragmentation and apoptotic bodies formation. The microautoradiographic study showed that 147Pm could infiltrate through cell membrane and displayed membrane-seeking condensation in cells. At the same time, the membrane-bounded apoptotic bodies were observed. Experimental results in recent study provide evidence that Molt-4 and Ana-1 immune cells undergo apoptosis while internally irradiated with 147Pm.

Key words: Fluorescence microscopy, Microautoradiography, Apoptosis, Molt-4 cell, Ana-l cell, Fission fragment 147Pm, Cell culture