Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 1997, Vol. 8 ›› Issue (4): 218

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Studies on structural features of human tumor necrosis factor

Yin Chuan-Yuan1, Guo Dong-Lin2, Xi Tao2, Xu Xian-Xiu2, Gu Qing-Chao3   

  1. 1 Department of Physics, Nanjing University, Nanjing 210093;
    2 National Laboratory of Pharmaceutic Biotechnology, Department of Biochemistry, Nanjing University, Nanjing 210093;
    2 Department of Polymer Science and Engineering, Nanjing University, Nanjing 210093
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Yin Chuan-Yuan, Guo Dong-Lin, Xi Tao, Xu Xian-Xiu, Gu Qing-Chao. Studies on structural features of human tumor necrosis factor.Nuclear Science and Techniques, 1997, 8(4): 218

Abstract: The mierostructure of human tumor necrosis factor alpha (TNF-α) and its mutant (TNF-b) has been investigated by utilizing positron annihilation lifetime spectroscopy, radioiodination of human TNF and L929 cells assay. The experimental results show that the long lifetime (τ2) and corresponding intensity (I2) of lower ortho-positronium annihilation in TNF-αa are longer and less than those in the TNF-b, respectively. It suggests that the TNF-b is smaller in free volume and higher in density than the TNF-α. The TNF-b may maintain a more favorable conformation for binding to TNF receptors, thus increasing its biological activity. It is then concluded that the increases in the cytotoxicity and in the density for the TNF-b result from the decreases in the free volurıne in the TNF-b.

Key words: Positron annihilation technique (PAT), Receptor binding assay, Tumor necrosis factor (TNF), Cytotoxicity