Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 1997, Vol. 8 ›› Issue (2): 107

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Cellular and molecular studies of mutation induction by low energy heavy ions

Tom K Hei1, Li X Zhu1, David J Chen2, Charles A Waldren3   

  1. 1 Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York 10032;
    2 The Genetics Group, Los Alamos National Laboratory, Los Alamos, NM 87545;
    3 Department of Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523
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Tom K Hei, Li X Zhu, David J Chen, Charles A Waldren. Cellular and molecular studies of mutation induction by low energy heavy ions.Nuclear Science and Techniques, 1997, 8(2): 107

Abstract: Mutation induction by low energy heavy ions was scored at the hypoxanthine guanine phosphoribosyl transferase (HGPRT) locus using both normal human fibroblasts and the human-hamster hybrid Ay cells. In addition, the mutation yield at a non-essential chromosome was also examined by using the S1 marker gene locating on human chromosome 11 in Ax cells. Mutagenicity induced by low energy heavy ions was dose and LET dependent. The induced mutant fractions at the S1 locus were consistently higher than those for HGPRT. Using a mutation system that can detect multilocus changes, it can be shown by either Southern blotting or multiplex PCR techniques that radiation can induce chromosomal deletions in the millions of basepairs.

Key words: Mutation, Molecular spectrum, Southern blot, PCR, Multilocus deletions