Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 1996, Vol. 7 ›› Issue (3): 151

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AFM studies of nonspecific binding of enzyme on DNA

Zhang Yi1 (张益), Xu Lei1 (徐磊), Ouyang Zhen-Qian1 (欧阳振乾), Chen Sheng-Fu1 (陈圣福), Sun Jie-Lin1 (孙洁林), Hu Jun1 (胡 钧), Li Min-Qian1 (李民乾), Xie Heng-Yue2 (谢恒月), Liu Zheng-Xia2 (刘征峡)   

  1. 1 Shanghai Institute of Nuclear Research, the Chinese Academy of Sciences, Shanghai 201800;
    2 Shanghai Institute of Cell Biology, the Chinese Academy of Sciences, Shanghai 200031
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Zhang Yi(张益), Xu Lei(徐磊), Ouyang Zhen-Qian(欧阳振乾), Chen Sheng-Fu(陈圣福), Sun Jie-Lin(孙洁林), Hu Jun(胡 钧), Li Min-Qian(李民乾), Xie Heng-Yue(谢恒月), Liu Zheng-Xia(刘征峡). AFM studies of nonspecific binding of enzyme on DNA.Nuclear Science and Techniques, 1996, 7(3): 151

Abstract: Atomic force microscope (AFM) is used to study restriction endonuclease digestion of plasmid DNA. pWRr plasmid DNA is digested by Hind III2and the specific and the nonspecific binding of the restriction endonuclease are imaged, and the biological function of the enzyme binding to nonspecific sites is discussed. In addition, it is found that nonspecific binding of Hind III could not induce the DNA charaéteristic bending angle.

Key words: Atomic ferce microscope, Plasmid, DNA, Restriction endonuclease, Nonspecific binding