Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2018 Impact factor 0.961

Nuclear Science and Techniques ›› 1995, Vol. 6 ›› Issue (3): 154

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LABELING McAb 3H11 AND ITS Fab FRAGMENT WITH 211At AND THEIR IMMUNOREACTIVITIES AND INJURY EFFECTS ON HUMAN GASTRIC CANCER CELLS

Liu Ning (刘 宁)1, Jin Jiannan (金建南)1, Zhang Shuyuan (张叔渊)1, Zhou Maolun (周懋伦)1, Wang Juan (王 娟)1, Luo Lin (罗 林)1, Wang Fangyuan (王方元)1, Luo Deyuan (罗德元)2, Wan Wenhui (万文徽)3 ,Dong Zhiwei (董志伟)3   

  1. 1 Institute of Nuclear Science and Technology, Sichuan Union University, Chengdu 610064;
    2 Institute of Cancer Research, West China University of Medical Sciences, Chengdu 610041;
    3 Beijing Institute for Cancer Research, Beijing 100034
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Liu Ning (刘 宁), Jin Jiannan (金建南), Zhang Shuyuan (张叔渊), Zhou Maolun (周懋伦), Wang Juan (王 娟), Luo Lin (罗 林), Wang Fangyuan (王方元), Luo Deyuan (罗德元), Wan Wenhui (万文徽) and Dong Zhiwei (董志伟). LABELING McAb 3H11 AND ITS Fab FRAGMENT WITH 211At AND THEIR IMMUNOREACTIVITIES AND INJURY EFFECTS ON HUMAN GASTRIC CANCER CELLS.Nuclear Science and Techniques, 1995, 6(3): 154

Abstract: An antigastric cancer monoclonal antibody, 3H11, and its Fab fragment, were labeled using p-(211At)-astatobenzoic acid (pAtBA) intermediate, in the yields of more than 30%. The results of in vitro  experiments show that 211At-3H11 and 211At-3H11 Fab are stable, and have specific immunoreactivities and cytotoxic effects to human gastric cancer cell M85. The cytotoxic effects are dependent on the concentration of 211At-3H11 or 211At-3H11 Fab and obviously stronger than that of Na211At.

Key words: 211At, Gastric cancer, Monoclonal antibody, Fab fragment, Labeling method, Immunoreactivity, Cytotoxic effect, In vitro