Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 1992, Vol. 3 ›› Issue (4): 268

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LABELLING OF METALLOTHIONEIN WITH 99mTc

Li Bizhong (李毕忠)1, Wu Yonghui (吴永慧)1, Liu Yuanfang (刘元方)1, Li Jun (李 军)1, Dong Yanbing (董燕兵)1, Wang Chongqing (王重庆)1, Ru Binggen (菇炳根)1, Han Bin (韩 斌) 2, Tian Jiahe(田嘉禾)2   

  1. 1 Peking University, Bei ing 100871, China;
    2 General Hospital of P.L.Army, Beijing 100853, China
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Li Bizhong (李毕忠), Wu Yonghui (吴永慧), Liu Yuanfang (刘元方), Li Jun (李 军), Dong Yanbing (董燕兵), Wang Chongqing (王重庆), Ru Binggen (菇炳根), Han Bin (韩 斌) , Tian Jiahe(田嘉禾). LABELLING OF METALLOTHIONEIN WITH 99mTc.Nuclear Science and Techniques, 1992, 3(4): 268

Abstract: 99mTc-Jabelled metallothionein (99mTc-MT) was prepared through both direct and transcomplexation labelling approaches. Buffer systems and a variety of other parameters in the direct labelling procedure were studied in detail, High radiolabelling yields of 93—96% and the specific radioactivity of 3.7 MBr/ug were obtained under optimal conditions by the direct labelling method. The prepared 99mTc-MT was stable in vitro. The chelate-exchange kinetics of MT with 99mTc-glucohepatonate and 99mTc-citrate were also studied. Biodistribution and imaging studies showed that 99mTc-MT was accumulated in the animal kidneys at an exceptionally high level, indicating that 99mTc -MT might be a potential renal imaging agent.

Key words: Metallothionein, Technetium-99m, Radiolabelling _Biodistribution, Imaging