Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 1992, Vol. 3 ›› Issue (4): 260

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CONFORMATION STUDY OF THE LARGE FRAGMENT OF E. COLI DNA POLYMERASE I BY STM

Yao Xiaowei(要小未)1, Li Minqian(李民乾)1, Xu Yaoliang(徐耀良)1, Gu Minming(顾敏明)1, Hu Jun(胡钧) Zhang Lanping(张兰平) Zhang Jiahua(张家骅)1, Lu Changde(陆长德)2, Qiu Minyan(裘敏燕)2   

  1. 1 Institute of Nuclear Research, Shanghai 201800, China;
    2 Institute of Biochemistry Research, Shanghai 200031, China
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Yao Xiaowei(要小未), Li Minqian(李民乾), Xu Yaoliang(徐耀良), Gu Minming(顾敏明), Hu Jun(胡钧) Zhang Lanping(张兰平) Zhang Jiahua(张家骅), Lu Changde(陆长德), Qiu Minyan(裘敏燕). CONFORMATION STUDY OF THE LARGE FRAGMENT OF E. COLI DNA POLYMERASE I BY STM.Nuclear Science and Techniques, 1992, 3(4): 260

Abstract: The large fragment of E.coli DNA polymerase I is imaged by scanning tunneling microscope. The specimen is deposited on highly oriented pyrolytic graphite surface, and then covered with pure paraffin oil in order to maintain hydration of the molecules. Images of the enzyme reveal an ellipsoid shape of 5.5--6.0 nm wide and 7.0 —7.5nm long. The conformation of the enzyme is in agreement with the model derived from X-ray crystallography studies.

Key words: STM, E. coli, DNA polymerase I