Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 1992, Vol. 3 ›› Issue (4): 244

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SOFT X一RAY IMAGES OF CHROMOSOMES WITHIN INTACT TUMOR CELLS TREATED WITH COLCHICINE

Wei Daoyan (魏道严)1, Shen Hengjia (沈恒嘉)1, Xu Wenxuan (徐文轩)2, Tang Esheng (唐鄂生)2   

  1. 1 Anhui Medical University, He fei 230032, China;
    2 Institute of High Energy Physics, Academia Sinica, Beijing 100080, China
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Wei Daoyan (魏道严), Shen Hengjia (沈恒嘉), Xu Wenxuan (徐文轩), Tang Esheng (唐鄂生). SOFT X一RAY IMAGES OF CHROMOSOMES WITHIN INTACT TUMOR CELLS TREATED WITH COLCHICINE.Nuclear Science and Techniques, 1992, 3(4): 244

Abstract: In order to avoid any possible effect of separating procedures from intact cell on morphologic structure of the chromosomes, cultivated Hep-2 tumor cells were treated with colchicine and observed by soft X-ray contact microscopy for the first time. The fine structures of chromosomes are more clear with stereo features. The thread—like and coarse granular structures twine and tangle up together within chromosome masses which can not easily be revealed in Wright's stained sample by light microscope or osmium stained sample by transmission electron microscope

Key words: Soft X-ray contact microscopy, Tumor cells, Colchicine, Chromosomes