Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2015, Vol. 26 ›› Issue (6): 060504 doi: 10.13538/j.1001-8042/nst.26.060504

• NUCLEAR PHYSICS AND INTERDISCIPLINARY RESEARCH • Previous Articles     Next Articles

A micro E-DNA sensor for selective detection of dopamine in presence of ascorbic acid

ZHU Dan,1, 2 LI Min, 1, 2 WANG Li-Hua , 1 ZUO Xiao-Lei 1   

  1. 1Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
    2University of Chinese Academy of Sciences, Beijing 100049, China
  • Contact: WANG Li-Hua, ZUO Xiao-Lei E-mail:wanglihua@sinap.ac.cn;zuoxiaolei@sinap.ac.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China (Nos. 31470960 and 21422508)

ZHU Dan, LI Min, WANG Li-Hua, ZUO Xiao-Lei . A micro E-DNA sensor for selective detection of dopamine in presence of ascorbic acid.Nuclear Science and Techniques, 2015, 26(6): 060504     doi: 10.13538/j.1001-8042/nst.26.060504
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Abstract:

In this paper, a novel method for selectively detection of dopamine (DA) in the interference of ascorbic acid (AA) is described. A nanometer-sized gold flower microelectrode (NGFME) is prepared by flame-etching and electrochemical deposition. The electrode tip was characterized by scanning electron microscope (SEM). The NGFME is sized at about 100 μm and dimensions of thorns of the electrode were in nanometers. By modifying with DA aptamer on the surface, the prepared aptasensor can selectively detect DA even in the presence of high concentration AA. Experimental results show that this NGFME has no response to AA. As a comparison, the carbon fiber electrode without DA aptamer modification is unable to effectively detect DA in the presence of AA. The NGFME is easy-to-prepare, selective and sensitive for DA detection down to 25 μM. The electrode can be expected to detect DA in vivo and in real biological samples.

Key words: Electrochemical sensor, Microelectrode, Dopamine detection, Ascorbic acid