Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2015, Vol. 26 ›› Issue (6): 060502 doi: 10.13538/j.1001-8042/nst.26.060502

• NUCLEAR PHYSICS AND INTERDISCIPLINARY RESEARCH • Previous Articles     Next Articles

A facile method for studying interaction of rhodamine B and bovine serum albumin: Towards physical-binding mediated fluorescence labeling of proteins

MA Yu-Xing,ZHONG Rui-Bo,GUO Jun,LIU Yu-Shuang,YUAN Ming,BAI Zhi-Jun,LIU Tao-Tao,ZHAO Xin-Min,ZHANG Feng   

  1. Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China
  • Contact: ZHANG Feng E-mail:fengzhang1978@hotmail.com
  • Supported by:

    Supported by the National Natural Science Foundation of China (Nos. 21171086 and 81160213), Inner Mongolia Autonomous Region science and Technology Department (No. 211-202077), Inner Mongolia Grassland Talent (No. 108-108038), Natural Science Foundation of Inner Mongolia Autonomous Region of China (No. 2013MS1121) and Inner Mongolia Agricultural University (Nos. 109-108040, 211-109003 and 211- 206038)

MA Yu-Xing, ZHONG Rui-Bo, GUO Jun, LIU Yu-Shuang, YUAN Ming, BAI Zhi-Jun, LIU Tao-Tao, ZHAO Xin-Min, ZHANG Feng. A facile method for studying interaction of rhodamine B and bovine serum albumin: Towards physical-binding mediated fluorescence labeling of proteins.Nuclear Science and Techniques, 2015, 26(6): 060502     doi: 10.13538/j.1001-8042/nst.26.060502

Abstract:

Strategies for labeling proteins with fluorophores are always important for biotechnology. Here we take a model protein (bovine serum albumin) and a typical fluorophore (rhodamine B) to demonstrate a direct labeling method just by physical adsorption. In combination with size exclusion chromatography and the Scartchard equation, we have developed a facile analysis method for calculating the binding constant and binding sites. The molecular docking method has been used to study the binding site in amino acid level.

Key words: Bovine serum albumin, Rhodamine B, Binding sites, Scatchard equation, Size exclusion chromatography