Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2015, Vol. 26 ›› Issue (5): 050303 doi: 10.13538/j.1001-8042/nst.26.050303

• NUCLEAR CHEMISTRY,RADIOCHEMISTRY,RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE • Previous Articles     Next Articles

Uranyl-cytochrome b5 interaction regulated by surface mutations and cytochrome c

SUN Mei-Hui,1 DU Ke-Jie,1 NIE Chang-Ming,1 WEN Ge-bo ,2 LIN Ying-Wu 1, 2   

  1. 1School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China
    2Laboratory of Protein Structure and Function, University of South China, Hengyang 421001, China
  • Supported by:

    Supported by National Natural Science Foundation of China (Nos. 21101091 and 11275090)

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SUN Mei-Hui, DU Ke-Jie, NIE Chang-Ming, WEN Ge-bo, LIN Ying-Wu . Uranyl-cytochrome b5 interaction regulated by surface mutations and cytochrome c.Nuclear Science and Techniques, 2015, 26(5): 050303     doi: 10.13538/j.1001-8042/nst.26.050303

Abstract:

Understanding uranium-protein interaction is important for revealing the mechanism of uranyl ion (UO2+ 2 ) toxicity. In this study, we investigated the interaction between UO2+ 2 and a quadruple mutant of cytochrome b5 (E44/48/56A/D60A cyt b5, namely 4A cyt b5) by spectroscopic approaches. The four mutated negativelycharged surface residues of cyt b5 have been considered to be the interactive sites with cytochrome c (cyt c). Also, we studied the interaction between UO2+ 2 and the protein-protein complex of 4A cyt b5-cyt c. The results were compared to the interaction between UO2+ 2 and cyt b5, and the interaction between cyt c and cyt b5-cyt c complex, from previous studies. It was found that the interaction of UO2+ 2 -cyt b5, i.e., uranyl ion binding to cyt b5 surface at Glu37 and Glu43 as previously proposed by molecular modeling, is regulated by both surface mutations of cyt b5 and its interacting protein partner cyt c. These provide valuable information on metal-protein-protein interactions and clues for understanding the mechanism of uranyl toxicity.

Key words: Uranium, Cytochrome, Heme protein, Protein-protein interaction, Fluorescence