Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2015, Vol. 26 ›› Issue (4): 040301 doi: 10.13538/j.1001-8042/nst.26.040301


Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry–99mTc labeling of graphene oxide nanosheets

JIANG Da-wei,1PENG Cheng,1SUN Yan-Hong,1 JIA Li-Na,1 LI Jian-Bo,2ZHANG Lan1   

  1. 1Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
    2Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, China
  • Contact: ZHANG Lan
  • Supported by:

    Supported by Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA02030000) and National Natural Science Foundation of China (Nos. 81360227 and 10875163)

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JIANG Da-wei, PENG Cheng, SUN Yan-Hong, JIA Li-Na, LI Jian-Bo, ZHANG Lan. Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry–99mTc labeling of graphene oxide nanosheets.Nuclear Science and Techniques, 2015, 26(4): 040301     doi: 10.13538/j.1001-8042/nst.26.040301


Graphene oxide (GO) nanosheets possess several advantages, such as a large surface, outstanding biocompatibility, and straightforward chemical modification capability. They also have great potential as a drugcarrier. In this article, we radiolabeled GO nanosheets with 99mTc, which satisfies the potential needs of micro- SPECT imaging probes in pre-clinical and clinical research. GO nanosheets were synthesized through the modified Hummers’ method, then GO nanosheets with azide group covalently functionalized in two steps were conjugated to DOTA (1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid) and functionalized with an alkynyl group by means of click chemistry. Then through the addition and reduction of technetium-99m, the 99mTc-DOTA-GO were attained. DOTA-conjugated GOs with lateral dimensions of 500–600 nm were synthesized. Both atomic force microscopy (AFM) and FT-IR were performed to characterize the GO-DOTA. Labeling efficiency of GO-DOTA with 99mTc was > 90% and radiochemical purities were > 96% with purification. We successfully synthesized graphene oxide derivatives, DOTA-conjugated GOs, via Click Chemistry, and it was labeled with 99mTc for SPECT imaging. High radiolabeling efficiency makes GO nanosheets suitable platforms for future molecular imaging research.

Key words: Graphene oxide nanosheets, 99mTc labeling, Click chemistry