Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2015, Vol. 26 ›› Issue (3): 030506 doi: 10.13538/j.1001-8042/nst.26.030506

• NUCLEAR PHYSICS AND INTERDISCIPLINARY RESEARCH • Previous Articles     Next Articles

Synchrotron radiation X-ray fluorescence analysis of Fe, Zn and Cu in mice brain associated with Parkinson’s disease

TIAN Tian,1, 2, 3 ZHANG Ji-Chao ,1, 2 LEI Hao-Zhi ,1, 2 ZHU Ying ,1, 2 SHI Ji-Ye ,4 HU Jun ,1, 2 HUANG Qing ,1, 2 FAN Chun-Hai ,1, 2 and SUN Yan-Hong 1, 2   

  1. 1Key Laboratory of Interfacial Physics and Technology, Chinese Academy of Sciences, Shanghai 201800, China
    2Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
    3University of Chinese Academy of Sciences, Beijing 100049, China
    4UCB Pharm, Slough SL1 3WE, UK
  • Contact: SUN Yan-Hong E-mail:sunyanhong@sinap.ac.cn
  • Supported by:

    Supported by the Ministry of Science and Technology of China (Nos. 2012CB825805 and 2012CB932600), National Natural Science Foundation of China (Nos. 11179004, 21390414, U1232113, U1232114, U1332119 and U1432116) and Youth Innovation Promotion Association of CAS

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TIAN Tian, ZHANG Ji-Chao, LEI Hao-Zhi, ZHU Ying, SHI Ji-Ye, HU Jun, HUANG Qing, FAN Chun-Hai, and SUN Yan-Hong . Synchrotron radiation X-ray fluorescence analysis of Fe, Zn and Cu in mice brain associated with Parkinson’s disease.Nuclear Science and Techniques, 2015, 26(3): 030506     doi: 10.13538/j.1001-8042/nst.26.030506

Abstract:

The contents and distributions of metal elements in the brain are closely related to neurodegenerative diseases. In this study, we examined Fe, Cu and Zn contents in the brain section associated with Parkinson‘s disease (PD) using synchrotron radiation X-ray fluorescence (SRXRF). PD mouse model induced by 1-methyl-4-phenyl- 1,2,3,6-terahydropyridine (MPTP) was used for the elemental analysis (e.g., Fe, Cu and Zn) in the substantia nigra pars compacta (SNpc) region of mice brain tissue samples. We found that mice in the MPTP group had higher contents of Fe, Cu and Zn in the SNpc than the control group. After treating the PD mice with rapamycin, the contents of Fe, Cu and Zn were reduced, the dopamine neurons and motor function were rescued correspondingly. The results prompted that the SRXRF provided an ideal method for tracing and analyzing the metal elements in the brain section to assess the pathological changes of PD model and the therapeutic effect of drugs.

Key words: Synchrotron radiation X-ray fluorescence, Metal element, Trace and analysis, Rapamycin, Parkinson’s diseases