Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2014, Vol. 25 ›› Issue (3): 030303 doi: 10.13538/j.1001-8042/nst.25.030303

• NUCLEAR CHEMISTRY,RADIOCHEMISTRY,RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE • Previous Articles     Next Articles

The role of autophagy in cell survival from heavy ion irradiation in the plateau region

XIA Jie-Fang, WANG Zhuan-Zi, LIU Qing-Fang, WEI Wei, ZHANG Dong-Zhi, LI Wen-Jian   

  1. 1School of Pharmacy, Lanzhou University, Lanzhou 730000, China
    2Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
    3Gansu Province People Hospital, Lanzhou 730000, China
  • Contact: WANG Zhuan-Zi E-mail:wangzz@impcas.ac.cn; wjli@impcas.ac.cn
  • Supported by:

    Supported by National Natural Science Foundation of China (Nos. 11105189, 11005131 and 11179002) and West Light Foundation of The Chinese Academy of Sciences (No. Y306010XBO)

XIA Jie-Fang, WANG Zhuan-Zi, LIU Qing-Fang, WEI Wei, ZHANG Dong-Zhi, LI Wen-Jian. The role of autophagy in cell survival from heavy ion irradiation in the plateau region.Nuclear Science and Techniques, 2014, 25(3): 030303     doi: 10.13538/j.1001-8042/nst.25.030303

Abstract:

To study cytotoxic effect of heavy ion irradiation in the plateau region, and investigate whether autophagy induced by heavy ion irradiation is cytoprotective, HeLa cells were irradiated with 350 MeV/u carbon ions beams, and the clonogenic survival was analyzed. The results showed that cell survival decreased with increasing doses. It was also found that G2/M-phase cells increased, and the autophagy-related activity was significantly high than the control. When autophagy was blocked by 3- methyladenine in carbon-ion irradiated cells, G2/M phase arrest and the percentage of apoptosis cells were further elevated, and cell survival decreased significantly, indicating the induction of cytoprotective autophagy by carbon-ion irradiation. Our results demonstrated that autophagy induced by carbon ion irradiation provided a self-protective mechanism in HeLa cells, short-time inhibition of autophagy before carbon-ion irradiation could enhance radiation cytotoxicity in HeLa cells.

Key words: Carbon ions irradiation, Cell survival, Apoptosis, Autophagy