Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2012, Vol. 23 ›› Issue (2): 97-102 doi: 10.13538/j.1001-8042/nst.23.97-102

• RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICIN • Previous Articles     Next Articles

Microarray screening of differentially expressed genes in DCX transfected U87 cells before and after irradiation

FENG Shuang1 WANG Jie1 JIANG Xin1 XU Yuanyuan1 ZHANG Haowen1 Cao Jianping1 YU Jiahua1 LIU Fenju1,*   

  1. 1School of Radiation Medicine and Protection, Jiangsu Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China
FENG Shuang, WANG Jie, JIANG Xin, XU Yuanyuan, ZHANG Haowen, Cao Jianping, YU Jiahua, LIU Fenju . Microarray screening of differentially expressed genes in DCX transfected U87 cells before and after irradiation.Nuclear Science and Techniques, 2012, 23(2): 97-102     doi: 10.13538/j.1001-8042/nst.23.97-102

Abstract:

Radiation therapy plays a critical role in the treatment of neurogliocytoma and it is known that doublecortin (DCX)-transfected U87 cells can inhibit tumor cell growth. Microarray analysis to screen for differentially expressed genes in DCX-transfected U87 cells before and after radiation uncovered DCX-related genes, the functions of DCX, and downstream genes in radiation therapy of neurogliocytoma. Stably transfected U87 cells were constructed (DCX-U87) and the differentially expressed genes were screened by microarray analysis to compare U87 cells with DCX-U87 cells in both non-irradiated and irradiated conditions. Cells were irradiated using 60Co γ-ray at a dose rate of 1.0 Gy/min. Mean values were subject to paired comparison analysis and genes with a p-value of less than 0.05 were analyzed. Differentially expressed genes can correlate with radiation sensitivity and DCX transfection. DCX and SPN proteins in DCX-U87 cells were detected by two groups of 0 and 10 Gy, but not the U87 cells, and their expression levels were higher in the 10 Gy group than in the 0 Gy group. The differential gene expression in DCX-U87 cells before and after radiation is helpful for future investigations into the mechanisms of radiation therapy in neurogliocytoma cells.

Key words: Microarray, DCX, U87, Ionizing radiation