Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2012, Vol. 23 ›› Issue (4): 247-251 doi: 10.13538/j.1001-8042/nst.23.247-251

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Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR GreenⅠ

CHEN Jiajia(1,2) ZHANG Chen(1,2) ZHAO Binyu(1,2) MI Lijuan1 ZHANG Yi1 HU Jun(1,)   

  1. 1 Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China 2 Graduate School of the Chinese Academy of Sciences,Beijing 100039,China
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CHEN Jiajia, ZHANG Chen, ZHAO Binyu, MI Lijuan, ZHANG Yi, HU Jun. Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR GreenⅠ.Nuclear Science and Techniques, 2012, 23(4): 247-251     doi: 10.13538/j.1001-8042/nst.23.247-251

Abstract:

Fluorescence enhancement in a DNA-dye system is favorable for sensitive and accurate DNA detection/ sensing technologies.In this paper,we report that the fluorescence of the double-stranded DNA(dsDNA) and SYBR GreenⅠ(SG) system(dsDNA-SG) can be effectively enhanced by negatively charged magnetic iron oxide (Fe_2O_3@DMSA) and gold nanoparticles in suitable concentrations,but positively charged nanoparticles quench the fluorescence.Effects of the Fe_2O_3@DMSA on the fluorescence intensities are investigated with dsDNA-SG of different lengths or complexities.The results show that nanoparticles perform similarly in enhancing fluorescence intensity for several kinds of dsDNA.However,the dsDNA concentration determines the fluorescence amplitude.It shows that fluorescence intensity of lower concentration dsDNA is enhanced remarkably in DNA-SG.The finding may be useful in sensitive biomolecular detection.

Key words: Surface charges, Fluorescence enhancement, Nanoparticles, Double-stranded DNA, SYBR GreenⅠ