Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2012, Vol. 23 ›› Issue (2): 124-128 doi: 10.13538/j.1001-8042/nst.23.124-128


Local and global sensitivity analysis of PCR kinetic model

LI Lanting1,2 MI Lijuan* HU Jun   

  1. 1Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Science, Shanghai 201800, China 2Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
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LI Lanting, MI Lijuan, HU Jun . Local and global sensitivity analysis of PCR kinetic model.Nuclear Science and Techniques, 2012, 23(2): 124-128     doi: 10.13538/j.1001-8042/nst.23.124-128


A mathematical model of polymerase chain reaction (PCR) containing uncertain and time-varying parameters has become important for model-based guidance experiment design. In this study, the local and global sensitivity analyses were conducted to identify that the responses of PCR process vary with their parameters of initial reactant concentrations and rate constants. Our results showed that the template concentration in initial reactant concentrations had the largest effect on DNA amplification yield. The rate constant characteristics showed that the local sensitivity basically determined the specific reactions; and the global sensitivity, the non-specific reactions. Our work should be helpful for optimizing PCR experimental conditions, and determining the PCR parameter sensitivities.

Key words: Polymerase chain reaction, Optimization, mathematical model, Sensitivity analysis