Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2012, Vol. 23 ›› Issue (1): 10-14 doi: 10.13538/j.1001-8042/nst.23.10-14

• SYNCHROTRON TECHNOLOGY AND APPLICATIONS • Previous Articles     Next Articles

In-line X-ray phase-contrast imaging of murine liver microvasculature ex vivo

LI Beilei1 XU Min1 WU Weizhong2 PENG Guanyun3 ZHANG Xi4 PENG Yifeng5 SHI Hongcheng1 CHEN Shaoliang 1   

  1. 1Department of Nuclear Medicine,Zhongshan Hospital Affiliated to Fudan University,Shanghai 200032,China 2Liver Cancer Institute,Zhongshan Hospital Affiliated to Fudan University,Shanghai 200032,China 3Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China 4Department of Radiology,Tumor Hospital Affiliated to Fudan University,Shanghai 200032,China 5Department of Radiology,No.10 People’s Hospital Affiliated to Tongji University,Shanghai 200072,China
LI Beilei, XU Min, WU Weizhong, PENG Guanyun, ZHANG Xi, PENG Yifeng, SHI Hongcheng, CHEN Shaoliang. In-line X-ray phase-contrast imaging of murine liver microvasculature ex vivo.Nuclear Science and Techniques, 2012, 23(1): 10-14     doi: 10.13538/j.1001-8042/nst.23.10-14

Abstract:

Imaging blood vessels is of importance for determining the vascular distribution of organs and tumors.Phase-contrast X-ray imaging can reveal the vessels in much more detail than conventional X-ray absorption method.Visualizing murine liver microvasculature ex vivo with phase-contrast X-ray imaging was performed at Shanghai Synchrotron Radiation Facility.Barium sulfate and physiological saline were used as contrast agents for the blood vessels.Blood vessels of <Φ20μm could be detected by replacing resident blood with physiological saline or barium sulfate.An entire branch of the portal vein (from the main axial portal vein to the ninth generation of branching) could be captured in a single phase-contrast image.It is demonstrated that selective angiography based on phase contrast X-ray imaging,with a physiological material of low Z elements (such as saline) being the contrast agent,is a viable imaging strategy.Further efforts will be focused on using the technique to image tumor angiogenesis.

Key words: Synchrotron radiation, X-ray, Phase-contrast imaging, Microvascular, Liver