Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2011, Vol. 22 ›› Issue (5): 293-298 doi: 10.13538/j.1001-8042/nst.22.293-298

• RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICIN • Previous Articles     Next Articles

Early monitoring of response to antimetabolic treatment of gemcitabine:A comparison of 18F-FLT and 18F-FDG uptake in Patu 8988 human pancreatic carcinoma cells

DENG Shengming ZHANG Bin~* WU Yiwei ZHANG Wei   

  1. Department of Nuclear Medicine,No.1 Hospital Affiliated to Soochow University,Suzhou 215006,China
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DENG Shengming, ZHANG Bin, WU Yiwei, ZHANG Wei . Early monitoring of response to antimetabolic treatment of gemcitabine:A comparison of 18F-FLT and 18F-FDG uptake in Patu 8988 human pancreatic carcinoma cells.Nuclear Science and Techniques, 2011, 22(5): 293-298     doi: 10.13538/j.1001-8042/nst.22.293-298

Abstract:

It is essential to predict the treatment efficacy of pancreatic carcinoma early.The purpose of this study was to examine whether ~(18)F-FDG(2'-deoxy-2'-[~(18)F]fiuoro-D-glucose) or ~(18)F-FLT(3'-deoxy-3'-~(18)F-fluorothymidine) PET can be used for chemosensitivity testing by investigating the binding characteristic of ~(18)F-FDG or ~(18)F-FLT with Patu 8988 human pancreatic carcinoma cell and the influence of gemcitabine in the uptake of ~(18)F-FDG or ~(18)F-FLT on Patu 8988.Under the conditions of 1×10~6 cells,3.7 kBq ~(18)F-FDG or ~(18)F-FLT,and incubation at 37℃for 100 min,the cell uptake of ~(18)F-FDG and ~(18)F-FLT was(60.60±3.05)%and(50.57±2.81)%,respectively.There was a significant decrease in TKl-LI(thymidine kinase 1 labeling index) 24 h after administration of gemcitabine.The uptakes of ~(18)F-FDG and ~(18)F-FLT were negatively correlated with the doses of gemcitabine(r= -0.928 for ~(18)F-FDG,r= -0.876 for ~(18)F-FLT,P<0.01).When same doses of gemcitabine were administered,the ~(18)F-FLT uptake inhibition rate was significantly higher than that of ~(18)F-FDG(P<0.01).These results indicate that the response to gemcitabine could be predicted as early as 24 h by ~(18)F-FDG or ~(18)F-FLT PET scans.~(18)F-FLT is more sensitive than ~(18)F-FDG to predict the response to therapy.

Key words: Pancreatic carcinoma, Uptake, Gemcitabine, (18)F-FDG, (18)F-FLT