Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2010, Vol. 21 ›› Issue (6): 357-361 doi: 10.13538/j.1001-8042/nst.21.357-361

• RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICIN • Previous Articles     Next Articles

Re-canalized stenostic rabbit nasolacrimal duct by the 125I radioactive probing:Affecting Bcl-2 and Bax protein expression

GUO Pengde~1 MA Qingjie~1 YANG Dongyan~1 GAO Shi~1 JIN Longyun~2 ZHOU Xueyan~1 CUI Qu~1 HAN Zhenguo~(1,*) ZHAO Guoqing~(1,*)   

  1. 1 China-Japan Union Hospital of Jilin University,Changchun 130033,China 2 Hong Qi Hospital of Mudanjiang Medical College,Mudanjiang 157011,China
GUO Pengde~ MA Qingjie, YANG Dongyan, GAO Shi, JIN Longyun, ZHOU Xueyan, CUI Qu, HAN Zhenguo, ZHAO Guoqing. Re-canalized stenostic rabbit nasolacrimal duct by the 125I radioactive probing:Affecting Bcl-2 and Bax protein expression.Nuclear Science and Techniques, 2010, 21(6): 357-361     doi: 10.13538/j.1001-8042/nst.21.357-361

Abstract:

To evaluate the ~(125)I radioactive probing re-canalizing stenostic nasolacrimal duct,the nasolacrimal duct stenosis models in epithelium and connective tissues are experimentally structured by inbred white rabbits(New Zealand),including the nasolacrimal duct stenosis,the mechanical probing with outer layer of thermoplastic tube,and the ~(125)I radioactive probing with the ~(125)I seeds sealing into the thermoplastic tube.After re-canalized for four weeks, tissue specimens from bilateral nasolacrimal ducts are obtained,and the Bcl-2 and Bax protein expression levels are evaluated by immunohistochemical staining analysis.Comparing with the blank control,the expression levels of the Bcl-2 and Bax in the nasolacrimal duct stenosis and the mechanical probing are significantly up-/down-regulated(p<0.05),but in the ~(125)I radioactive probing are down-/up-regulated(p<0.05) and can be used to re-canalize the stenostic lacrimal passage.The results show that the ~(125)I radioactive probing is a therapeutical mechanism for radioactive probing strategy for treating nasolacrimal duct stenosis to induce cell apoptosis.

Key words: Lacrimal passage, Radiation, Bcl-2 and Bax, (125)I radioactive probing