Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2010, Vol. 21 ›› Issue (6): 347-351 doi: 10.13538/j.1001-8042/nst.21.347-351

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Total DNA of Glycyrrhiza uralensis transformed into Hansenula anomala by ion implantation:Preparing Glycyrrhizic acid in recombined yeasts

JIN Xiang~1 L(U|¨)Jie~(1,3) MA Yuan~2 MAO Peihong~(1,*)   

  1. 1 Center of Ion Beam Biotechnology,College of Physics and Technology,Xinjiang University,Urumqi 830008,China 2 College of Resource and Environment Science,Xinjiang University,Urumqi 830046,China 3 College of Life Science and Pharmacy,Nanjing University of Technology,Nanjing 210009,China
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JIN Xiang, L(U, ¨)Jie, MA Yuan, MAO Peihong. Total DNA of Glycyrrhiza uralensis transformed into Hansenula anomala by ion implantation:Preparing Glycyrrhizic acid in recombined yeasts.Nuclear Science and Techniques, 2010, 21(6): 347-351     doi: 10.13538/j.1001-8042/nst.21.347-351

Abstract:

Glycyrrhizic acid(GA) in Glycyrrhiza uralensis(G.uralensis) is physiologically active.In this study,the total DNA of wild G.uralensis was randomly transformed into Hansenula anomala by implantation of low-energy Ar~+ and N~+,to produce five recombinant yeast strains relating to biological synthesis of the GA or Glycyrrhetinic acid (GAs).After culturing in liquid medium for 96 h,the resultant GA,18α-GAs and 18β-Gas were determined by reversed-phase high performance liquid chromatography(RP-HPLC),and the corresponding concentrations were 114.49,0.56,and 0.81 mg·L~(-1).After one hundred primers were analyzed with random amplified polymorphic DNA (RAPD),the seven different DNA fragments were produced by the N7059 strain of recombined yeasts,and,the polymerase chain reaction(PCR) verified that one of them came from the genome of G.uralensis,indicating a successful transfer of genetic information by ion implantation.

Key words: Ion implantation, total DNA of G.uralensis, Transformation, Recombinant yeast, Glycyrrhizic acid