Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2013, Vol. 24 ›› Issue (6): 060101 doi: 10.13538/j.1001-8042/nst.2013.06.001


Observation of cavitation and water-refilling processes in plants with X-ray phase contrast microscopy

XUE Yanling XIAO Tiqiao* DU Guohao TONG Yajun LIU Huiqiang DENG Biao XIE Honglan XU Hongjie*   

  1. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Zhangjiang Campus, Shanghai 201203, China
  • Contact: XIAO Tiqiao, XU Hongjie;
  • Supported by:

    Supported by the National Natural Science Foundation of China (No.11105213), the State Key Development Program for Basic Research of China (No.2010CB834301), the External Cooperation Program of the Chinese Academy of Sciences (No.GJHZ09058), and the Knowledge Innovation Program of the Chinese Academy of Sciences.

XUE Yanling, XIAO Tiqiao, DU Guohao, TONG Yajun, LIU Huiqiang. Observation of cavitation and water-refilling processes in plants with X-ray phase contrast microscopy.Nuclear Science and Techniques, 2013, 24(6): 060101     doi: 10.13538/j.1001-8042/nst.2013.06.001


With the spatial coherence of X-rays and high flux and brightness of the 3rd generation synchrotron radiation facility, X-ray phase contrast microscopy (XPCM) at Shanghai Synchrotron Radiation Facility (SSRF) can provide high resolution dynamic imaging of low electron density materials in principle. In this paper, we investigated the cavitation and water-refilling processes in rice and bamboo leaves utilizing XPCM at SSRF. The occurrence of xylem cavitation was recorded in vivo. The study also revealed that under different dehydration conditions, cavitation occurs in different degrees, and therefore, the refilling process is different. The results demonstrate that SSRF can provide high enough fluxes to study dynamic processes in plants in real-time, and XPCM is expected to be a promising method to reveal the mechanisms of cavitation and its repair in plants nondestructively.

Key words: X-ray phase contrast microscopy, Synchrotron radiation, Cavitation/embolism, Water transportation