Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2013, Vol. 24 ›› Issue (4): 040302 doi: 10.13538/j.1001-8042/nst.2013.04.006


99mTc-3P4-RGD2 radiotracers for SPECT/CT of esophageal tumor

GAO Shi1  MA Qingjie1  WEN Qiang1  JIA Bing2  LIU Zhaofei2#br# CHEN Zuowei3  ZHANG Haishan1,*  LI Dandan1,*   

  1. 1China-Japan Union Hospital, Jilin University, Changchun 130033, China
    2Medical Isotopes Research Center, Peking University, Beijing 100191, China
    3Department of Nuclear Medicine, People’s Hospital of Shenzhen, Shenzhen 518020, China
  • Received:2013-01-08
  • Contact: ZHANG Haishan, LI Dandan,
  • Supported by:

    Supported by National Natural Science Foundation of China (NSFC) projects (No.81271606) and Research Fund of Science and Technology Department of Jilin Province (No. 201015185 and No. 201201041) and the Research Fund of Shenzhen Sci-tech Department of Guangdong Province (No.201102154)

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GAO Shi, MA Qingjie, WEN Qiang, JIA Bing, LIU Zhaofei, CHEN Zuowei, ZHANG Haishan, LI Dandan. 99mTc-3P4-RGD2 radiotracers for SPECT/CT of esophageal tumor.Nuclear Science and Techniques, 2013, 24(4): 040302     doi: 10.13538/j.1001-8042/nst.2013.04.006


In recent years, several RGD (Arg-Gly-Asp)-based radiotracers have already been successfully tested in human for the visualization of integrin αvβ3, demonstrating its feasibility in tumor diagnosis. In this paper, we evaluated the 99mTc-3P4-RGD2 single photon emission computed tomography/computerized tomography(SPECT/CT)in patients suffering from space occupying disease of esophagus. 40 patients (34 males and 6 female; mean age: 58.3 years) with a suspected space occupying lesion of esophagus were included, thus finally obtaining their definite pathologic diagnosis (malignant, n=32; benign, n=8). All patients underwent endoscopic, barium esophagography and SPECT/CT imaging preoperatively. The chest SPECT was performed at 4 h after administration of 99mTc-3P4-RGD2 with a dose of 939±118 MBq. The diagnosis precision, sensitivity and specificity among these methods were compared. The relationship between radioactiveuptake and clinical pathological stage of esophageal carcinoma was discussed by calculating the tumor to normal esophagus (T/N). Meanwhile, the integrin αvβ3 expression was assessed immunohistochemically in postoperative esophageal tissues. 31 patients were diagnosed as esophageal carcinoma; and 1, leiomyosarcoma; and 6. leimyoma; and 2, esophageal tuberculosis. The accuracy, sensitivity and specificity of barium esophagography, endoscopic and SPECT/CT imaging are 92.5/93.8/87.5%, 97.5/96.9/100%, and 90/90.6/87.5%, respectively. Abnormal accumulation of radiotracer in 29 malignant lesions is observed. The SPECT/CT imaging displayed the region of radioactive uptake and lesions matched extremely with the T/N ratio from 1.31 to 2.79 (mean 2.04). A case with pulmonary metastases and a case with mediastinal lymph node metastases were found which were missed by barium esophagography and endoscopic. The 99mTc-3P4-RGD2 uptake of the esophageal carcinoma masses had no relevance to the tumor pathologic classification (P>0.05). There was a significant positive correlation between T/N ratio and positive cell percentage of integrin αvβ3 (r=0.976), demonstrating the certain clinical potential in the diagnosis of esophageal carcinoma.

Key words:  99mTc-3P4-RGD2, Esophageal tumor, SPECT, Integrin αvβ3