Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2013, Vol. 24 ›› Issue (3): 030504 doi: 10.13538/j.1001-8042/nst.2013.03.006


Mechanical force-induced DNA damage during AFM single-molecule manipulation

ZHANG Chen1,2  DUAN Na1,2  DAI Bin1,2  ZHANG Yi1  ZHANG Donghua1  HU Jun1   

  1. 1Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Jiading Campus, Shanghai 201800, China
    2University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2013-04-01
  • Contact: HU Jun
  • Supported by:

    Supported by National Basic Research Program of China (973 Program Nos. 2007CB936000 and 2012CB932600), National Natural Science Foundation of China (No. 21073222) and Chinese Academy of Sciences (No. KJCX2-EW-N03)

ZHANG Chen, DUAN Na, DAI Bin, ZHANG Yi, ZHANG Donghua, HU Jun. Mechanical force-induced DNA damage during AFM single-molecule manipulation.Nuclear Science and Techniques, 2013, 24(3): 030504     doi: 10.13538/j.1001-8042/nst.2013.03.006


Many environmental factors can cause DNA damage, such as radiation, heat, oxygen free radical, etc., which can induce mutation during DNA replication. Meanwhile, DNA molecules are subjected to various mechanical forces in numerous biological processes. However, it is unknown whether the mechanical force would induce DNA damage and introduce mutation during DNA replication. With the combination of single-molecule manipulation based on atomic force microscopy (AFM), single molecular polymerase chain reaction (SM-PCR) and Sanger’s sequencing, we investigated the effect of mechanical force on DNA. The results show that mechanical force can cause DNA damage and induce DNA mutation during amplification.


Key words: Manipulation, Atomic force microscope, Polymerase chain reaction (PCR), DNA damage and mutation