Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2013, Vol. 24 ›› Issue (3): 030304 doi: 10.13538/j.1001-8042/nst.2013.03.003

• NUCLEAR CHEMISTRY,RADIOCHEMISTRY,RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE • Previous Articles     Next Articles

N-acetylcysteine protect lymphocytes and cytokines against heavy ion irradiation via counteracting the glutamate

XIE Yi1,2,3  ZHANG Hong1,2,3  WANG Yipei4  LIU Yang1,2,3  CHEN Yingtai5   

  1. 1Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
    2Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China
    3Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China
    4Department of Chemistry & Biochemistry, University of Delaware, Newark, DE 19711, USA
    5Department of Abdominal Surgical Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China
  • Received:2012-12-12
  • Contact: ZHANG Hong E-mail:zhangh@impcas.ac.cn
  • Supported by:

    Supported by National Basic Research Program (973 Program) (No. 2010CB834202), National Natural Science Foundation of China (No. 10835011), Key Scientific Technology Research Projects of Gansu Province (Nos. 0702NKDA045 and 0801NKDA001) and West Light Foundation (Doctor aid, 2009)

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XIE Yi, ZHANG Hong, WANG Yipei, LIU Yang, CHEN Yingtai. N-acetylcysteine protect lymphocytes and cytokines against heavy ion irradiation via counteracting the glutamate.Nuclear Science and Techniques, 2013, 24(3): 030304     doi: 10.13538/j.1001-8042/nst.2013.03.003

Abstract:

We evaluated the protective effect of N-acetylcysteine (NAC) on immunity system irradiated by 12C6+ ion beam. Kun-Ming mice were whole-body irradiated by 12C6+ ion at doses of 0, 0.5, 1, 1.5, 2, 2.5 and 3 Gy. The results showed that in saline group, the lymphocytes DNA double-strand breaks (DSBs), maleic dialdehyde, thymocytes number in G0/G1 and apoptosis percentage increased with dose increment, and the levels of interferon-γ, glutathione, superoxide radical (SOD) and natural killer cells activity decreased with dose increment. However, there were no significant changes in NAC-treated group. The data indicated that pre-treatment with NAC could significantly remove the ROS by counteracting the glutamate, decrease excessive lipid peroxidation reaction and SOD damages, and protect DNA, lymphocytes and cytokines against irradiation.

Key words: N-acetylcysteine, 12C6+ heavy ion, DNA damagesOxidative stress, Lymphocytes and cytokines