Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2013, Vol. 24 ›› Issue (1): 010301 doi: 10.13538/j.1001-8042/nst.2013.01.001

• NUCLEAR CHEMISTRY,RADIOCHEMISTRY,RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE • Previous Articles     Next Articles

Damage of DNA ends induced by mechanical force during AFM nano-manipulation

DUAN Na, WANG Xinyan, ZHANG Chen, ZHANG Yi, HU Jun   

  1. 1Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
    2University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-09-21
  • Supported by:

    Supported by the Major State Basic Research Development Program in China (No.2007CB936000);Chinese Academy of Sciences (No. KJCX2-EW-N03);National Science Foundation of China (Nos.10975175, 90923002, 21073222 and 10874198)

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DUAN Na, WANG Xinyan, ZHANG Chen, ZHANG Yi, HU Jun. Damage of DNA ends induced by mechanical force during AFM nano-manipulation.Nuclear Science and Techniques, 2013, 24(1): 010301     doi: 10.13538/j.1001-8042/nst.2013.01.001

Abstract:

An experimental and statistical study was carried out to explore the effects of mechanical forces on the ends of linear double-stranded DNA (dsDNA) fragments. Mechanical force was applied onto individual DNA molecules during atomic force microscope (AFM)-based picking-up manipulation. By comparing the PCR efficiency of two DNA fragments with primers either at ends or at the inner regions, it was found that the ends of DNA fragments were damaged during picking-up process.

Key words: Nanomanipulation, DNA damage, Mechanical force, Atomic force microscope (AFM)