Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2009, Vol. 20 ›› Issue (2): 87-92 doi: 10.13538/j.1001-8042/nst.20.87-92

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Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation

LI Ning1,2,3 ZHANG Hong1,2,* WANG Yanling1,2,3 WANG Xiaohu4 HAO Jifang1,2   

  1. 1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2 Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China 3 Graduate School of Chinese Academy of Sciences, Beijing 100039, China 4 Department of Radiotherapy, Gansu Tumor Hospital, Lanzhou 730050, China
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LI Ning, ZHANG Hong, WANG Yanling, WANG Xiaohu, HAO Jifang. Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation.Nuclear Science and Techniques, 2009, 20(2): 87-92     doi: 10.13538/j.1001-8042/nst.20.87-92

Abstract:

In this paper, we study the ability of DNA-PK-deficient (M059J) and -proficient (M059K) cells to undergo the rate of cellular proliferation, cell cycle distribution and apoptosis after 10 Gy X-ray irradiation, and the role of DNA-PK in radiosensitivity. The results showed that M059J cells exhibited hyper-radiosensitivity compared with M059K cells. A strong G2 phase arrest was observed in M059J cells post irradiation. Significant accumulation in the G2 phase in M059J cells was accompanied by apoptosis at 12 h. Altogether, the data suggested that DNA-PK may have two roles in mammalian cells after DNA damage, a role in DNA DSB repair and a second role in DNA-damaged cells to traverse a G2 checkpoint, by which DNA-PK may affect cellular sensitivity to ionizing radiation.

Key words: DNA-dependent protein kinase (DNA-PK), Cell cycle checkpoint, Apoptosis, Radiosensitivity