Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2009, Vol. 20 ›› Issue (6): 331-334 doi: 10.13538/j.1001-8042/nst.20.331-334


Proteomic analysis of the gene DR1709 mutant in Deinococcus radiodurans

TIAN Shuangqi QIN Guangyong LI Yongliang CHANG Shenghe*   

  1. Ion Beam Bio-engineering Lab, School of Physics, Zhengzhou University, Zhengzhou 450052, China
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TIAN Shuangqi, QIN Guangyong, LI Yongliang, CHANG Shenghe. Proteomic analysis of the gene DR1709 mutant in Deinococcus radiodurans.Nuclear Science and Techniques, 2009, 20(6): 331-334     doi: 10.13538/j.1001-8042/nst.20.331-334


DR1709 is a predicted Mn2+ transporter in Deinococcus radiodurans(D.radiodurans). The mensuration method to evaluate protein viability with two-dimensional electrophoresis in D.radiodurans and the mutants was established in this study. The results showed that after DR1709 was disrupted, the expressions of DR1120 (acetokinase), DR1691 (heat shock protein), DR1485 (putative lipase), DR2095 (putative c-type cytochrome) and other three hypothetical proteins (DR0124, DR0047 and DR2474) were repressed. However the expression of DR1794 (putative nosX) was induced. Phenomena above suggested that the increased radiation-sensitivity of the mutant cells may be attributed to not only the protection of gene DR1709, but also the proteins’ different expressions between the wild type and the mutant might also play important roles in protecting D.radiodurans from irradiation. Although DR2095 was a homologue of c-type cytochrome, it has no realitic functions.

Key words: Deinococcus radiodurans, DR1709, Proteomics