Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2009, Vol. 20 ›› Issue (5): 286-291 doi: 10.13538/j.1001-8042/nst.20.286-291

• RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICIN • Previous Articles     Next Articles

Analysis of secretome of breast cancer cell line with an optimized semi-shotgun method

TANG Xiaorong1,2 YAO Ling1,2 CHEN Keying1,2 HU Xiaofang1,2 XU Lisa X.1,2,3,* FAN Chunhai4   

  1. 1Key Laboratory of Systems Biomedicine (Shanghai Jiao Tong University), China Ministry of Education, Shanghai, 200240, China 2Dept. of Biomedical Engineering, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China 3Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200240, China 4Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China
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TANG Xiaorong, YAO Ling, CHEN Keying, HU Xiaofang. Analysis of secretome of breast cancer cell line with an optimized semi-shotgun method.Nuclear Science and Techniques, 2009, 20(5): 286-291     doi: 10.13538/j.1001-8042/nst.20.286-291

Abstract:

Secretome, the totality of secreted proteins, is viewed as a promising pool of candidate cancer biomarkers. Simple and reliable methods for identifying secreted proteins are highly desired. We used an optimized semi-shotgun liquid chromatography followed by tandem mass spectrometry (LC-MS/MS) method to analyze the secretome of breast cancer cell line MDA-MB-231. A total of 464 proteins were identified. About 63% of the proteins were classified as secreted proteins, including many promising breast cancer biomarkers, which were thought to be correlated with tumorigenesis, tumor development and metastasis. These results suggest that the optimized method may be a powerful strategy for cell line secretome profiling, and can be used to find potential cancer biomarkers with great clinical significance.

Key words: Secretome, Semi-shotgun, Breast cancer