Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2009, Vol. 20 ›› Issue (5): 271-276 doi: 10.13538/j.1001-8042/nst.20.271-276

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Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

LÜ Jie1,2 MAO Peihong2 JIN Xiang2 YU Long1 YING Hanjie1,*   

  1. 1 College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 210009 Nanjing, China 2 Center of Ion Beam Biotechnology, College of Physics Science and Technology, Xinjiang University, 830008 Urumqi, China
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Lü Jie, MAO Peihong, JIN Xiang, YU Long, YING Hanjie. Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01.Nuclear Science and Techniques, 2009, 20(5): 271-276     doi: 10.13538/j.1001-8042/nst.20.271-276

Abstract:

Bacillus subtilis Bac01 was mutated by 15 keV N+ ions of 1.5×1016 cm-2. The mutant strain Bac11 with high yield of endoglucanase was isolated using carboxymethylcellulose sodium and congo red indicative plates. It exhibited higher endoglucanase activitiy (381.89IU) than the original strain Bac01 (93.33IU). Two 1,500 bp endoglucanase gene fragments were obtained with PCR amplification from B. subtilis Bac01 and mutant strain Bac11. BLAST comparison result indicated that 10 nucleotides mutated. Bioinformatics methods were used to analyze the two predicted amino acid sequences, and it was found that 5 amino acid residues changed, being all in the cellulose-binding domain of endoglucanase.

Key words: Ion irradiation, Bacillus subtilis, Endo-1,4-β-D-glucanase, Activity, Gene mutation