Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2009, Vol. 20 ›› Issue (3): 157-162 doi: 10.13538/j.1001-8042/nst.20.157-162

• RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICIN • Previous Articles     Next Articles

Preparation, quality control and biodistribution of [61Cu]-doxorubicin for PET imaging

JALILIAN Amir Reza1,* YOUSEFNIA Hassan2 FAGHIHI Reza2 AKHLAGHI Mehdi1 ZANDI Hassan1   

  1. 1Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School, Karaj, Iran 2Department of Nuclear Engineering, School of Engineering, Shiraz University, Shiraz, Iran
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JALILIAN Amir Reza, YOUSEFNIA Hassan, FAGHIHI Reza. Preparation, quality control and biodistribution of [61Cu]-doxorubicin for PET imaging.Nuclear Science and Techniques, 2009, 20(3): 157-162     doi: 10.13538/j.1001-8042/nst.20.157-162

Abstract:

This work was conducted for radiolabeling of an anticancer antibiotic, i.e. doxorubicin with 61Cu for production of possible tracer used in PET oncology. 61Cu was prepared with natural zinc target and 22 MeV150 ?A protons via natZn(p, xn)61Cu reaction with a yield of 123.2 MBq·μA–1·h–1. Optimization reactions were performed for pH, temperature and concentration. Biodistribution of the tracer was studied in normal and fibrosarcoma bearing mice. At the optimized conditions, ITLC showed that radiochemical purity was over 97% with a specific activity of 2.22× 103MBq ·mmol-1·L-1. This was kept unchanged even with presence of human serum as well as room temperature for 5 h. Biodistribution of the tracer in fibrosarcoma bearing mice demonstrated significant tumor uptake after 2 h. This tracer can be used in the detection of various tumors responding to doxorubicin chemotherapy using PET scan and/or determination of tumor therapy response to doxorubicin chemotherapy.

Key words: Copper-61, Doxorubicin, Radiolabeling, Biodistribution