Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2008, Vol. 19 ›› Issue (5): 290 doi: 10.1016/S1001-8042(09)60006-3

• RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICIN • Previous Articles     Next Articles

The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line

GAO Xiuli1,Δ YANG Shunfang2,*,Δ YU Yongli1,* SHI Meiping3 ZHAO Lanxiang3 YE Jianding4 LU Jianying5   

  1. 1 Department of Nuclear Medicine, Shanghai No.6 Hospita Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China
    2 Department of Nuclear Medicine, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China
    3 Department of Pathology, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China
    4 Department of Radiology, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China
    5 Department of Prenatal Diagnosis, Maternal and Child Health Hospital Affiliated to Tongji University, Shanghai 200040,China
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GAO Xiuli YANG Shunfang YU Yongli SHI Meiping ZHAO Lanxiang YE Jianding LU Jianying. The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line.Nuclear Science and Techniques, 2008, 19(5): 290     doi: 10.1016/S1001-8042(09)60006-3
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Abstract: The study was to investigate the role of pinhole single photon emission computed tomography (SPECT), the human pulmonary adenocarcinoma bone-seeking metastasis cell line SPC-A-1BM was used. These cells form typical osteolytic bone metastases when inoculated into the arterial circulation of NIH-Beige-Nude-XD (BNX) mice via the left ventricle. In order to evaluate the irradiation impact of 99mTc-MDP versus X-ray on cells growth, we used six groups of SPC-A-1BM cells in our imaging scheme and irradiated by various doses of 99mTc-MDP (37, 74, 111, 370, 740 MBq) and X-ray(40 kV, 2 mA, 6 s) respectively. The cell’s number of each group was well recorded in different exposure time(4, 8, 12, 24, 48, 72, 96 hours). After that, SPC-A-1BM cells (1×106) were inoculated into the mice via left ventricle. We compared the results obtained with those different doses of 99mTc-MDP using pinhole SPECT and conventional X-ray skeletal surveys. The data show that the cell-survival number of 111 MBq group has insignificant difference with that of X-ray and the dose is adequate to have an ideal image. Besides, it is important that the chromosome of the cells in the group of 111 MBq showed no irradiation-related damages in our test. These results implied that 99mTc-MDP pinhole SPECT may provide another way other than conventional X-ray skeletal surveys in detecting bone metastasis of pulmonary adenocarcinoma in BNX mice.

Key words: 99mTc-MDP, Pinhole SPECT, Radiation, Pulmonary adenocarcinoma, Bone metastasis, Animal model