Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2018 Impact factor 0.961

Nuclear Science and Techniques ›› 2008, Vol. 19 ›› Issue (3): 138 doi: 10.1016/S1001-8042(08)60039-1


Assessment of biological changes in wheat seedlings induced by 12C6+-ion irradiation

ZHANG Luwei1,2,3 ZHANG Hong2 ZHANG Xiaofu1 ZHU Jianlan1,4,*   

  1. 1College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China
    2 Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
    3 Key Laboratory of Grassland-Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou 730070, China
    4Sino-US Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China
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ZHANG Luwei ZHANG Hong ZHANG Xiaofu ZHU Jianlan . Assessment of biological changes in wheat seedlings induced by 12C6+-ion irradiation.Nuclear Science and Techniques, 2008, 19(3): 138     doi: 10.1016/S1001-8042(08)60039-1

Abstract: The present study was designed to evaluate the effect of 12C6+ ion beam (10~80 Gy) on biological changes of wheat seedlings. Reactive oxygen species (ROS)-related biomarkers and the quantification of plant survival and growth were examined at 10 day after carbon ions irradiation (LET: 30.8 keV/μm). The results showed that heavy ions obviously enhanced ROSs reflected by the production of O2- and H2O2 as well as TBARS, and treatment with 20 Gy achieved the peak value, suggesting that higher mutagenic potential may occur at 20 Gy. Simultaneously, increase of SOD activity was induced by heavy ions to counteract ROS accumulation. On the other hand, higher doses at 40 and 80 Gy inhibited wheat growth and survival in comparison with the control, and reversely lower doses at 10 or 20 Gy stimulated wheat growth and survival. In conclusion, the above observations imply that a dose range of 20~40 Gy is likely promised for wheat mutation breeding.

Key words: 12C6+ ion, Wheat, Reactive oxygen species, Plant growth, Plant survival