Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2008, Vol. 19 ›› Issue (2): 105 doi: 10.1016/S1001-8042(08)60032-9


Feasibility of 99mTc-TRODAT-1 Micro-SPECT imaging of dopamine transporter in animal retinas

ZHAO Juan1 QI Yujin2 DAI Qiusheng2 ZHANG Xuezhu2 QU Xiaomei3 HUANG Jia3 LIU Xingdang1,*   

  1. 1 Department of Nuclear Medicine, Huashan Hospital of Fudan University, Shanghai 200040, China
    2 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
    3 Eye and ENT Hospital of Fudan University, Shanghai 200031, China
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ZHAO Juan QI Yujin DAI Qiusheng ZHANG Xuezhu QU Xiaomei HUANG Jia LIU Xingdang . Feasibility of 99mTc-TRODAT-1 Micro-SPECT imaging of dopamine transporter in animal retinas.Nuclear Science and Techniques, 2008, 19(2): 105     doi: 10.1016/S1001-8042(08)60032-9

Abstract: In this paper, 99mTc-TRODAT-1 Micro-SPECT (single-photon emission computed tomography) was used for imaging dopamine transporter (DAT) in retinas and to investigate the changes of DAT in retinas of guinea pigs with form deprivation myopia. Pigmented guinea pigs aged 3 weeks were devided into form deprivation myopia (FDM) group (n=6) and normal control group (n=6). The test group wore translucent goggles randomly for 4 weeks, and both groups underwent biometric measurement (refraction and axial length) before and after the experiment. Micro-SPECT retinas imaging was performed at the 4th week after injection of 99mTc-TRODAT-1. The retinas were clearly resolved in the images. The ratio of 99mTc-TRODAT-1 uptake in the myopic retinas (11.55±2.80) was 3.64±1.40 lower than that in the control eye (15.20±1.98), and 2.35±1.05 lower than that in the fellow eyes (13.90±2.04). The results showed that 99mTc-TRODAT-1 Micro-SPECT eye imaging can be used to trace the distribution and changes of DAT in retina, and DAT in the myopic retinas were lower than that in the normal control eyes and fellow eyes. Micro-SPECT may provide a new approach for further studies on the role of dopamine system in the experimental myopia.

Key words: Micro-SPECT, 99mTc-TRODAT-1, Dopamine transporter, Form deprivation myopia, Guinea pig