Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2007, Vol. 18 ›› Issue (5): 282 doi: 10.1016/S1001-8042(07)60062-1

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Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells

ZHOU Qingming1,2 ZHANG Hong1,* ZHANG Xingxia3   

  1. 1 Institute of Modern Physics , Chinese Academy of Sciences , Lanzhou 730000,China;
    2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China;
    3 The First Hospital of Lanzhou University, Lanzhou 730000,China
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ZHOU Qingming ZHANG Hong ZHANG Xingxia. Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells.Nuclear Science and Techniques, 2007, 18(5): 282     doi: 10.1016/S1001-8042(07)60062-1
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Abstract: To investigate effects of nitric oxide on cellular radio-sensitivity, three human glioma cell lines, i.e. A172, A172 transfected green fluorescence protein (EGFP) gene (EA172) and A172 transfected inducible nitric oxide syn-thesis (iNOS) gene (iA172), were irradiated by 12C6+ ions to 0, 1 or 2Gy. Productions of nitric oxide and glutathione (GSH) in A172, EA172 and iA172 were determined by chemical methods, cell cycle was analyzed by flow cytometry at the 24th hour after irradiation, and survival fraction of the cells was measured by colorimetric MTT assay at the 5th day after irradiation. The results showed that the concentrations of nitric oxide and GSH in iA172 were significantly higher than in A172 and EA172; the G2/M stage arrest induced by the 12C6+ ion irradiation was observed in A172 and EA172 but not in iA172 at the 24th hour after exposure; and the survival fraction of iA172 was higher than that of EA172 and iA172. Data suggest that the radio-sensitivity of the A172 was reduced after the iNOS gene transfection. The increase of GSH production and the change of cellular signals such as the cell cycle control induced by nitric ox-ide may be involved in this radio-resistance.

Key words: Nitric oxide, 12C6+ heavy ion, A172 human glioma cells, Radio-resistance