Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2006, Vol. 17 ›› Issue (5): 272 doi: 10.1016/S1001-8042(06)60050-X


Study of calf thymus DNA irradiated in vitro with MeV fluorine ions

MIAO Qi1,2,* LAI Jiang-Nan2 XUE Jian-Ming2 QIN Huai-Li2 WANG Wei-Dong1 QIN Guang-Yong1 WANG Yu-Gang2   

  1. 1Henan Provincial Key Lab of Ionbeam Bioengineering, Zhengzhou University, Zhengzhou 450052, China;
    2Key Laboratory of Heavy Ion Physics, Peking University, MOE, Beijing 100871, China
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MIAO Qi LAI Jiang-Nan XUE Jian-Ming QIN Huai-Li WANG Wei-Dong QIN Guang-Yong WANG Yu-Gang. Study of calf thymus DNA irradiated in vitro with MeV fluorine ions.Nuclear Science and Techniques, 2006, 17(5): 272     doi: 10.1016/S1001-8042(06)60050-X

Abstract: A study of the fragments of DNA irradiated with MeV ions is important for the understanding of the DNA damage mechanism and the subsequent biological effects (induced by heavy ions). In this experiment, the products of calf thymus DNA (CT DNA) irradiated with MeV fluorine ions were analyzed using agarose gel electrophoresis, modified time-of-flight mass spectrometer (MALDI-TOF), and high-performance liquid chromatography (HPLC). The results showed that the molecular mass of the fragments were concentrated around 831 bp with agarose gel electrophoresis, there was no observable product in the range of 1,000  30,000 (m/q) using MALDI-TOF, and small biomolecules were separated from the products. The results of this study indicated that the strand breaks of calf thymus DNA induced by MeV fluorine ions were nonrandom.

Key words: MeV ion, Irradiation, DNA, Damage