Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2020, Vol. 31 ›› Issue (10): 96 doi: 10.1007/s41365-020-00807-5

• ACCELERATOR, RAY AND APPLICATIONS •     Next Articles

Characterization of metal element distributions in the rat brain following ischemic stroke by synchrotron radiation microfluorescence analysis

Shu-Peng Shi1 • Hui Wang1 • Zhuo-Hui Chen1 • Xiao-Han Li1 • Shi-Xin Liu1 • Meng-Qi Zhang1,2   

  1. 1 Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China
    2 National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China
  • Received:2020-04-24 Revised:2020-08-16 Accepted:2020-08-20
  • Contact: Meng-Qi Zhang E-mail:zhangmengqi8912@163.com
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (No. 81501025) and the Natural Science Foundation of Hunan Province (Nos. 2020JJ4134 and 2016JJ3174).
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Shu-Peng Shi, Hui Wang, Zhuo-Hui Chen, Xiao-Han Li, Shi-Xin Liu, Meng-Qi Zhang. Characterization of metal element distributions in the rat brain following ischemic stroke by synchrotron radiation microfluorescence analysis.Nuclear Science and Techniques, 2020, 31(10): 96     doi: 10.1007/s41365-020-00807-5
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Abstract: Ischemic stroke is one of the leading causes of death worldwide, and effective treatment strategies in the chronic phase of this disease remain insufficient. Homeostasis of metals in the brain plays an important role in maintaining normal brain function. However, the dynamic spatial distributions of iron, zinc, calcium, potassium, and copper in a rat brain following ischemic stroke and the association between structural distribution and function remain to be elucidated. In this study, we used a synchrotron radiation-based micro-X-ray fluorescence technique to image element mapping changes in special rat brain regions after ischemic stroke, showing the distribution characteristics of iron, zinc, calcium, potassium, and copper. We demonstrated, for the first time, the consistent dynamic spatial distributions of metal elements at a series of time points (3 h, 4.5 h, 6 h, 12 h, 1 d, 3 d, 5 d, 7 d, 10 d, 14 d, 28 d) after brain ischemia, which revealed that the homeostasis of iron, zinc, calcium, potassium, and copper in the brain was disturbed with distinctive change trends, providing clear insights in understanding the underlying pathogenesis of stroke from a novel perspective, thus laying the foundation of further developing new drug targets for stroke treatment.

Key words: Ischemic stroke, Synchrotron radiation, X-ray fluorescence, Metal, Rat brain